RESUMO
Amplification of the 16S rRNA gene from a blood sample obtained from a dog in southeastern Brazil was used to confirm a naturally acquired Ehrlichia (E.) canis infection. Following isolation and culturing of the new bacterial strain called Uberlandia, partial sequences of the dsb and p28 genes were obtained. The dsb partial sequence of the novel strain was 100% similar to dsb gene sequences of E. canis obtained from different geographic areas around the world. Conversely, the p28 partial sequence for the E. canis Uberlândia strain differed at several nucleotides from other sequences available in GenBank. To confirm the antigenic profile of the Uberlândia strain, an indirect immunofluorescence assay against E. canis antigens was performed using dog sera collected from two different areas in Brazil (Uberlândia and São Paulo). The results suggest that both antigens were able to identify animals seropositive for E. canis in Brazil since these Brazilian strains appear to be highly conserved.
Assuntos
Antígenos de Bactérias , Proteínas de Bactérias/genética , Doenças do Cão/microbiologia , Ehrlichia canis/genética , Ehrlichia canis/imunologia , Ehrlichiose/veterinária , Animais , Antígenos de Bactérias/sangue , Proteínas da Membrana Bacteriana Externa/genética , Proteínas da Membrana Bacteriana Externa/metabolismo , Proteínas de Bactérias/metabolismo , Sequência de Bases , Brasil , Doenças do Cão/diagnóstico , Cães , Ehrlichia canis/isolamento & purificação , Ehrlichiose/diagnóstico , Ehrlichiose/microbiologia , Técnica Indireta de Fluorescência para Anticorpo/veterinária , Masculino , Dados de Sequência Molecular , Reação em Cadeia da Polimerase/veterinária , RNA Ribossômico 16S/genética , RNA Ribossômico 16S/metabolismo , Alinhamento de Sequência/veterináriaRESUMO
Ehrlichia canis, etiologic agent of Canine Monocytic Ehrlichiosis, is an obligatory intracellular bacterium that parasitizes monocytes and macrophages. In this study we analyzed the role of the cytoskeleton specifically actin microfilaments and microtubules, components of inositol phospholipid signaling pathway such as phospholipase C (PLC), protein kinase (PTK) and calcium channels as well as the role of iron in the E. canis proliferation in DH82 cells. Different inhibitory compounds were used for each component: Cytochalasin D (inhibits actin polymerization), Nocodazole (inhibits microtubule polymerization), Neomycin (PLC inhibitor), Genistein (PTK inhibitor), Verapamil (calcium channel blocker) and Deferoxamine (iron chelator). We observed a significant decrease in the total number of bacteria in infected cells treated suggesting that these cellular components analized are essentials to E. canis proliferation.
Assuntos
Citoesqueleto de Actina/efeitos dos fármacos , Doenças do Cão/microbiologia , Ehrlichia canis/crescimento & desenvolvimento , Ehrlichiose/veterinária , Microtúbulos/efeitos dos fármacos , Transdução de Sinais , Citoesqueleto de Actina/metabolismo , Animais , Canais de Cálcio/metabolismo , Linhagem Celular , Doenças do Cão/metabolismo , Cães , Ehrlichiose/metabolismo , Ehrlichiose/microbiologia , Inositol/metabolismo , Ferro/metabolismo , Microscopia de Fluorescência/veterinária , Microtúbulos/metabolismo , Proteínas Quinases/metabolismo , Fosfolipases Tipo C/metabolismoRESUMO
Degenerative progressive hereditary mieloencefalopathy is a disease described in young Brown Swiss cattle. In these animals the disease occurs in calves between six months and two years old showing mainly neurological signs. Two Zebu Gir calves were examined at the Veterinary Hospital of Uberaba, both with balance deficits. The neurological examination has shown that they were mentally alert, with appropriate conscience, head tremor, normal sensitive reflexes, normal sensitivity for superficial and deep pain, motor deficit of the pelvic limbs with severe ataxia. The calves were sacrificed. Based on the clinical case and necroscopic and histopathological findings, it could be suggested that the neuropathy, which affected both animals described above, is very similar to the degenerative progressive hereditary mieloencefalopathy observed in Brown Swiss calves.
A mieloencefalopatia degenerativa progressiva hereditária é uma doença descrita em bovinos Pardo Suíço. Nesses animais, a doença ocorre em bezerros de seis meses a dois anos de idade, e os principais sinais clínicos são nervosos. Dois bezerros Gir foram atendidos no Hospital Veterinário de Uberaba, ambos com dificuldades de equilíbrio motor. O exame clínico revelou estado mental alerta, consciência apropriada, tremores de cabeça, reflexos sensitivos normais, sensibilidade à dor profunda e superficial normal, déficit motor dos membros posteriores com intensa incoordenação. Os bezerros foram sacrificados. Baseado no quadro clínico e nos achados necroscópicos e histopatológicos, pode-se sugerir que a neuropatia que acometeu os dois animais descritos em muito se assemelha à mieloencefalopatia degenerativa progressiva hereditária, observada em bovinos da raça Pardo Suíço.
